Ie-Ming Shih, MD, PhD

Ie-Ming Shih, MD, PhD

About Project

Applying DREAMing to detect epigenetic markers in ovarian cancer


DREAM in this context stands for Discrimination of Rare EpiAlleles by Melt. It is a technique that can pluck out the signals of extremely rare structural genetic mutations associated with ovarian cancer from the sea of noise of normal DNA in which they reside. Dr. Shih has an extremely specific technique for detecting mutated DNA sequences associated with ovarian cancer. This high specificity means that almost all of the signals it detects come from ovarian cancer – resulting in a low false positive rate. Although it is highly specific, it is not very sensitive, meaning that it does not pick up all of the signals that are there – resulting in a high false negative rate. Coupling this extant specific technique with DREAM, which detects structural alterations in DNA with high sensitivity, will hopefully improve the sensitivity of the sequencing method to generate an overall more accurate test for the early detection of ovarian cancer.

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